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A Lecithinase from the Amoeba Hartmannella rhysodes *
Author(s) -
ELSON CHARLES,
GEYER ROBERT P.,
CHANG ROBERT S.
Publication year - 1970
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1970.tb04711.x
Subject(s) - lecithinase , microbiology and biotechnology , toxin , enzyme , strain (injury) , biology , amoeba (genus) , biochemistry , chemistry , anatomy
SYNOPSIS A lipogenic toxin produced by the amoeba Hartmannella rhysodes , Fernald strain, made mammalian cells in culture round up and fill with fat droplets. From this toxin an enzyme was obtained with lecithinase and lysolecithinase activities. This enzyme is different from any isolated elsewhere. When purified enzyme was added to strain L mouse fibroblasts in culture, the cells rounded up and became fatty. Much of the lipogenic activity of the original toxin can be ascribed to this enzyme.