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Synchronization of the Cell Cycle of Tetrahymena by Starvation and Refeeding *
Author(s) -
CAMERON I. L.,
JETER J. R.
Publication year - 1970
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1970.tb04708.x
Subject(s) - tetrahymena , starvation , synchronization (alternating current) , biology , microbiology and biotechnology , computer science , endocrinology , telecommunications , channel (broadcasting)
SYNOPSIS The present report describes a simple and useful method for synchronizing mass cultures of the ciliate Tetrahymena pyriformis. The method employs a nutritional approach which involves starvation of the cells in a non‐nutrient phosphate buffer followed by refeeding with an enriched nutritional growth medium. It takes 240 minutes after refeeding before the first cells start to divide. Radioautographic and DNA determinations taken together show that starved cells are stalled in the GI nuclear DNA condition and that essentially all of the cells replicate their DNA prior to their first cell division.