Premium
Chlorophyll Formation in Euglena gracilis var. bacillaris: Effect of Chelating Agents
Author(s) -
DUBASH P. J.,
REGE D. V.
Publication year - 1970
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1970.tb02383.x
Subject(s) - euglena gracilis , ethylenediaminetetraacetic acid , chelation , euglena , chemistry , chlorophyll , metal ions in aqueous solution , intracellular , nuclear chemistry , biochemistry , metal , botany , biology , chloroplast , inorganic chemistry , organic chemistry , gene
SYNOPSIS. 8‐Hydroxyquinoline (8‐HQ) and cysteine markedly inhibit the synthesis of chlorophylls in non‐proliferating etiolated Euglena gracilis var. bacillaris on illumination. This is thought to be due, at least to some extent, to the binding of Mg 2+ ions in the non‐proliferation medium, but largely as a consequence of intracellular chelating action. The effect of 8‐HQ could be reversed by the presence of metal ions higher in the Mellor‐Malley series than Mg 2+ , e.g., Zn 2+ and Co 2+ . Sodium diethyldithiocarbamate (Na‐DDG) and ethylenediaminetetraacetic acid (EDTA) have no effect on chlorophyll synthesis in non‐proliferation conditions. Growth in the light is more affected than growth in the dark by all these chelating agents, including Na‐DDG and EDTA. This is due to the differential requirement of Mg 2+ under these 2 growth conditions.