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Effect of Acetaldehyde on Growth in Succinate Media and Labeling RNA with 14 C Succinate in Polytomella caeca
Author(s) -
WISE DONALD L.
Publication year - 1970
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1970.tb02353.x
Subject(s) - acetaldehyde , chemistry , biochemistry , rna , succinates , ethanol , gene
SYNOPSIS. Acetaldehyde had been shown to prevent growth of Polytomella caeca in acetate media when present at 4 mM and to depress labeling of RNA with acetate‐2‐ 14 C. In order to avoid inhibition by acetaldehyde of possible malate synthase activity believed necessary for incorporation of acetate, succinate was substituted for acetate. Acetaldehyde had the same effect on growth in succinate media and labeling of RNA with succinate‐2‐ 14 C as it had on utilization of acetate.