Premium
Prolonged Survival of Tetrahymena at 0.5 C in Citrated, Lecithinized, Defined Media
Author(s) -
COX DUDLEY
Publication year - 1970
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1970.tb02345.x
Subject(s) - lecithin , tetrahymena pyriformis , casein , chemistry , chemically defined medium , biochemistry , hydrolysate , uridine , biology , food science , tetrahymena , andrology , hydrolysis , in vitro , rna , medicine , gene
SYNOPSIS. A fully defined medium aside, also one with acid‐hydrolyzed casein, both pH 6.2–6.4 permitted survival of Tetrahymena pyriformis (syngen 1, mating type II) for no more than a week at 0.5 C, despite fortification with crude soy lecithin. Lecithin had permitted prolonged survival in crude media (pH 7.2), probably due largely to its phytosterols and antioxidants. Metal imbalances appeared responsible for the poor survival in defined media because additional citrate, histidine or Fe permitted monthlong survival. Al or Ni in the presence of increased citrate were also favorable. The casein‐hydrolysate medium and a fully defined medium, both fortified with lecithin, and with additional citrate or Fe, permitted survival at 0.5 C for at least 2 months. Krebs‐cycle intermediates, uridine, thymidine, adenosine, and guanosine acted as growth substrates in nearly carbohydrate‐free media, which suggests trial of their survival‐promoting properties for cold‐stored cultures. Parallels are noted for survival in the cold between Tetrahymena and erythrocytes and sperm.