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Inhibition of Deoxyribonuclease Activity in Synchronized Tetrahymena
Author(s) -
HOLM BORIS J.
Publication year - 1969
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1969.tb02324.x
Subject(s) - tetrahymena pyriformis , deoxyribonuclease , puromycin , deoxyribonuclease i , tetrahymena , enzyme , deoxyribonucleases , dna , biochemistry , substrate (aquarium) , dna synthesis , biology , enzyme assay , chemistry , specific activity , microbiology and biotechnology , protein biosynthesis , base sequence , ecology
SYNOPSIS. Deoxyribonuclease (DNase) activity in crude homogenates of synchronized cultures of Tetrahymena pyriformis GL was inhibited by added acidic polymers (which inactivated the enzyme) and polyamines (which blocked enzyme sites on the substrate). The inhibition appeared to be a nonspecific reaction between basic groups on the enzyme and acidic groups on the substrate. The relation between variation in DNase activity and amount of DNA was analyzed by means of puromycin and mitomycin C. DNase activity was lower after addition of each antibiotic, without significant change in amount of DNA. The results suggest that induction of DNase synthesis is governed by the amount of DNA.