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Gamma Irradiation of Stentor polymorphus
Author(s) -
PREECE ALAN W.
Publication year - 1969
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1969.tb02271.x
Subject(s) - irradiation , cell division , division (mathematics) , gamma irradiation , oxygen , radiochemistry , biology , cell , fission , chemistry , biochemistry , physics , mathematics , organic chemistry , nuclear physics , arithmetic , quantum mechanics , neutron
SYNOPSIS.Stentor polymorphus was irradiated with 60 Co gamma rays at 525 rads/minute to examine the effect on survival, cell division, oral membranellar frequency and oxygen uptake. Both survival and cell division were studied on single cells. The LD 50 is 285 kilorads but cell division is inhibited below this dose; 110 kilorads doubles the 1st post‐irradiation division interval, and a delay of 240 hours occurs at the maximum tolerated dose of 310 kilorads. Conjugating cells are more sensitive, with an LD 50 of approximately 40 kilorads. The frequency of cilia in the membranellar band, measured stroboscopically, is reduced by 30% after 14.5 kilorads, and stopped by higher doses. Recovery has a similar time scale to recovery of fission. A Clarke electrode was used to measure changes in oxygen uptake after irradiation. Depressions of up to 50% were found. Recovery followed a similar pattern to that of ciliary activity and cell division.