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Giant Amoebae from Colorado Identified by Transplantation Tolerance 1
Author(s) -
DANIELS EDWARD W.,
SUSTARSIC PATRICIA A.,
McCLELLAN J. FORBES
Publication year - 1969
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1969.tb02252.x
Subject(s) - amoeba (genus) , biology , protozoa , transplantation , amoeba proteus , myotis lucifugus , protoplasm , botany , zoology , cytoplasm , medicine , surgery , microbiology and biotechnology
SYNOPSIS. Giant multinucleated amoebae, discovered in Colorado near Ft. Collins and tentatively identified as Pelomyxa carolinensis , were successfully cultured in a manner identical with that used for P. carolinensis. The ultrastructure of the Colorado amoebae, in comparison with that of other large amoebae, was like that of P. carolinensis , but different from those of Amoeba proteus, P. illinoisensis , and P. palustris. Protoplasmic grafts between the Colorado amoebae and P. carolinensis were then exchanged by microsurgery. These grafts were well tolerated, and the recipient amoebae reproduced at a rate comparable to that of non‐grafted controls. Other Colorado amoebae received grafts from P. illinoisensis , but all recipients died without cell division a few days after microsurgery. These nutritional, cytologic and transplantation‐tolerance data reveal that the amoebae from Colorado are P. carolinensis.