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Differentiation and Multiplication of Dyskinetoplastic Trypanosoma cruzi in Tissue Culture and in the Mammalian Host *
Author(s) -
DEANE MARIA P.,
KLOETZEL JUDITH K.
Publication year - 1969
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1969.tb02242.x
Subject(s) - trypanosoma cruzi , multiplication (music) , host (biology) , biology , tissue culture , virology , parasite hosting , genetics , in vitro , computer science , world wide web , physics , acoustics
SYNOPSIS. In cultures of the Y strain of Trypanosoma cruzi treated with acriflavine, a very high proportion of the crithidiae may be dyskinetoplastic. These crithidiae cannot be maintained in subcultures but are able to differentiate into (dyskinetoplastic) metatrypanosomes. In tissue cultures infected with these metatrypanosomes, or with blood trypanosomes and treated with acriflavine, dyskinetoplastic T. cruzi is able to go thru the whole sequence of stages that characterizes its cycle in the vertebrate host: penetration by trypanosomes into cells, differentiation into leishmaniae, multiplication in this phase and differentiation again into trypanosomes. The same may occur in the mammalian host itself. The physiological implications of these findings are discussed and special attention is called to polymorphism of blood forms of T. cruzi , which probably has the same significance it has in the brucei‐evansi group of trypanosomes.