Premium
The DNA of Acanthamoeba spp.; A Method for Extraction and Its Characterization
Author(s) -
ADAM KATHERINE M. G.,
BLEWETT D. A.,
FLAMM W. G.
Publication year - 1969
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1969.tb02225.x
Subject(s) - dna , dna extraction , acanthamoeba , biology , extraction (chemistry) , buoyant density , mitochondrial dna , yield (engineering) , component (thermodynamics) , chemistry , chromatography , microbiology and biotechnology , genetics , polymerase chain reaction , materials science , gene , physics , metallurgy , thermodynamics
SYNOPSIS. A method is described which permits the recovery of a high yield of undegraded DNA from Acanthamoeba castellanii. The DNA of A. castellanii is comprised of 2 species: the buoyant density of the major component is 1.720 and of the minor component, 1.693. The renaturation characteristics of the minor component suggest that it is of mitochondrial origin. Two components are also present in the DNA of A. palestinensis, A. polyphaga and A. astronyxis. The buoyant densities of major and minor components differ between species.