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Growth of Tetrahymena in Carbohydate‐Free High‐Glutamate Media
Author(s) -
COX DUDLEY,
FRANK OSCAR,
HUTNER S. H.,
BAKER HERMAN
Publication year - 1968
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1968.tb02199.x
Subject(s) - tetrahymena , proline , glutamic acid , glycerol , substrate (aquarium) , biochemistry , chemically defined medium , chemistry , glutamate receptor , strain (injury) , carbohydrate , serine , biology , food science , amino acid , enzyme , anatomy , ecology , in vitro , receptor
SYNOPSIS. In chemically defined media, pH 6.4–6.8, supplementation with glycerol permitted growth equal to that with a carbohydrate supplement. In a pH 5.4 medium described in detail, containing L‐glutamic acid 0.25%, supplementation with L‐serine (essential for this strain), especially when further supplemented with proline, likewise permitted growth nearly equal to that with carbohydrate. These results are discussed in relation to the question of which components of peptone act as substrates and the resemblances of the Tetrahymena pattern of substrate utilization to that of metazoa.