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Oxygen Consumption of Coccidial Sporozoites During in Vitro Excystation
Author(s) -
VETTERLING JOHN M.
Publication year - 1968
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1968.tb02167.x
Subject(s) - incubation , oxygen , in vitro , respiration , eimeria acervulina , biology , chemistry , microbiology and biotechnology , biochemistry , eimeria , anatomy , organic chemistry
SYNOPSIS. Oxygen consumption of sporozoites of Eimeria acervulina, E. necatrix , and E. meleagrimitis was determined during in vitro excystation. Increase in O 2 consumption occurred only when the permeability of the oocyst membrane was altered by either grinding or incubation in cystine buffer with CO 2 when excystation fluid was present. Addition of 10 −3 M KCN completely inhibited respiration. The O 2 consumption reached a peak during the time the sporozoites were excysting, and then decreased to a lower level where it remained steady while the sporozoites were awaiting stimulus to penetrate host cells.