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Isolation, Characterization and Synthesis of DNA from a Malaria Parasite *
Author(s) -
WALSH CHARLES J.,
SHERMAN IRWIN W.
Publication year - 1968
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1968.tb02163.x
Subject(s) - parasite hosting , dna , biology , dna synthesis , cytosine , microbiology and biotechnology , guanine , dna replication , isolation (microbiology) , plasmodium (life cycle) , biochemistry , nucleotide , world wide web , computer science , gene
SYNOPSIS. Methods for the isolation and purification of DNA from the intraerythrocytic stages of the avian malaria parasite Plasmodium lophurae are described. The DNA of P. lophurae was found to have a 19 mole‐percent guanine plus cytosine (G+C) composition as determined by melting temperature and density measurements in CsCl gradients, whereas that of the host cell nucleus was 35 molepercent G+C. Synthesis of DNA by P. lophurae was studied by following the incorporation of P 32 during the in vitro growth of plasmodia in infected cell suspension cultures. DNA synthesis was linear during the multiple nuclear divisions of a single growth cycle in highly synchronous cultures. Separation of P 32 labeled parasite DNA from the DNA of the duck erythrocyte on CsCl gradients showed that only parasite DNA was synthesized.