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Mating Type Determination in Stock 51, Syngen 4 of Paramecium aurelia Grown in Axenic Culture *
Author(s) -
BUTZEL HENRY M.
Publication year - 1968
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1968.tb02124.x
Subject(s) - paramecium aurelia , axenic culture , axenic , mating type , biology , cytoplasm , mating , paramecium , genetics , zoology , botany , microbiology and biotechnology , bacteria , gene
SYNOPSIS. The use of axenic medium permits the study of mating type determination in stock 51 (sensitive) of syngen 4 of Paramecium aurelia. A high frequency of cytoplasmically bridged pairs was correlated with a high frequency of change of mating type following conjugation in axenic medium. The direction of change was predominantly from mating type VII to mating type VIII, suggesting a dominance of type VIII cytoplasm in the clones arising from a mixed cytoplasmic ancestry. No significant effect of either lower temperature or of N a X 3 upon the pattern of mating type determination was found. The high frequency of cytoplasmic bridges between conjugants led to the formation of many double or higher multiplex clones.