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Action of Sulfanilamide on Ochromonas malhamensis
Author(s) -
POTTY V. H.,
TAMHANE D. V.
Publication year - 1968
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1968.tb02120.x
Subject(s) - sulfanilamide , growth inhibition , methionine , in vitro , chemistry , folic acid , vitamin , biochemistry , amino acid , medicine
SYNOPSIS. Sulfanilamide inhibited the growth of O. malhamensis. Sulfanilamide growth inhibition was reversed competitively by PABA and by very high concentrations of folic acid. Folic acid at low concentrations, however, accentuated sulfa inhibition of growth. Vitamin B 12 , methionine, p ‐aminobenzoylglutamic acid and pteroic add were effective to some extent as antagonists of sulfa. A marked reduction in the folate synthesis was accompanied by sulfa growth inhibition. This was restored on growth restoration by PABA, folic acid, vitamin B 12 and methionine. The reduction in folate synthesis held for all the folate fractions except one derivative—a formyl poly‐glutamate. Sulfanilamide‐inhibited cells had a considerable activity for in vitro synthesis of folate activity from precursors. ∼75% activity being retained at the 90% growth inhibition level. There was no change in chlorophyll, RNA and DNA contents as a result of sulfa growth inhibition.