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The Leishmania‐Leptomonad Transformation of Leishmania donovani : Nutritional Requirements, Respiration Changes and Antigenic Changes *
Author(s) -
SIMPSON LARRY
Publication year - 1968
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1968.tb02112.x
Subject(s) - biology , puromycin , biochemistry , leishmania donovani , transformation (genetics) , respiration , antigen , in vitro , incubation , protein biosynthesis , immunology , leishmaniasis , botany , visceral leishmaniasis , gene
SYNOPSIS. The leishmania‐leptomonad transformation of Leishmania donovani occurs in 20–40 hr in vitro at 27 C in the absence of cell division. The rate and extent of the process depend on the available nutrients. A source of amino adds and glucose (or sucrose) are required. Several basic or acidic amino acids can substitute for all amino acids present in the complete medium. Leishmanial forms freshly isolated from hamster spleen have a QO 2 (μM O 2 /min/10 7 cells) of 0.29 ± 0.01 at 27 C in phosphate buffered saline with glucose. This respiration is sensitive to KCN, antimycin A, and Na amytal, and hence is probably cytochrome dependent. The QO 2 remains constant for 5–6 hr and then increases 5–7 fold by 20–30 hr of incubation at 27 C in the proper medium. The transformation is inhibited in terms of morphogenesis and increase in respiration by Actinomycin D, puromycin and mitomycin C. Appearance of new soluble antigens during the transformation was found by gel diffusion and immunoelectrophoresis.