Premium
Structure and Function of Mitochondria in Crithidia fasciculata *
Author(s) -
HILL GEORGE C.,
BROWN CHARLES A.,
CLARK MARTIN V.
Publication year - 1968
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1968.tb02093.x
Subject(s) - crithidia fasciculata , mitochondrion , cytochrome c oxidase , electron transport chain , alternative oxidase , biochemistry , cytochrome , antimycin a , biology , respiration , biogenesis , mitochondrial biogenesis , chemistry , microbiology and biotechnology , biophysics , enzyme , anatomy , gene
SYNOPSIS. In correlating mitochondrial structure with composition and function of the electron‐transport system in Crithidia fasciculata , failure to find cytochrome oxidase in isolated mitochondria coincided with the presence of longitudinally‐oriented lamellar cristae in the mitochondria in intact cells. Cytochromes b and c were detected spectrophotometrically. Respiration of intact cells and mitochondria, measured polarographically, was sensitive to 10 −4 M antimycin A and 5 × 10 −4 M KCN. The difficulty in detecting cytochrome oxidase and the biogenesis of mitochondria are briefly discussed.