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Freeze‐Preservation of Leucocytozoon simondi Sporozoites in Liquid Nitrogen
Author(s) -
KOCAN RICHARD M.,
KELKER NORMAN E.,
CLARK DAVID T.
Publication year - 1967
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1967.tb02067.x
Subject(s) - liquid nitrogen , gametocyte , parasite hosting , liquid phase , nitrogen , biology , chemistry , andrology , immunology , malaria , medicine , physics , plasmodium falciparum , organic chemistry , world wide web , computer science , thermodynamics
SYNOPSIS. Sporozoites of L. simondi were maintained in a viable state for 7 months in liquid nitrogen. Comparison of parasite development initiated with fresh and frozen sporozoites showed a delay in development of each stage studied. Comparisons of prepatency, first elongate gametocytes, peak density of round and elongate forms, anemia and disappearance of megaloschizonts were made. In each phase there was a delay of 2–3 days in ducks infected with frozen sporozoites.