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A Method for the Mass Collection of Axenically Cultivated Paramecium aurelia *
Author(s) -
SOLDO A. T.,
WAGTENDONK W. J.
Publication year - 1967
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1967.tb02035.x
Subject(s) - paramecium aurelia , paramecium , biology , microbiology and biotechnology
SYNOPSIS. A method is described for the collection of large numbers of axenically cultivated Paramecium aurelia free of contaminating particulate debris. The procedure takes advantage of the fact that the ciliates are negatively geotropic and involves migrating the organisms directly from the culture medium into an overlayer of salt solution of low density. Details are given for the construction of a simple apparatus used to carry out these migrations.