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A Method for the Isolation of Macronuclei from Paramecium aurelia
Author(s) -
STEVENSON I.
Publication year - 1967
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1967.tb02018.x
Subject(s) - paramecium aurelia , paramecium , rna , biology , dna , biochemistry , gene
SYNOPSIS. A method is described for the isolation of macronuclei from Paramecium aurelia. It has also been successfully employed with Didinium nasutum. The yield is up to 50% with P. aurelia. Some results on the DNA, RNA and protein contents are given. The isolated macronuclei of P. aurelia are able to incorporate ATP and UTP into acid‐insoluble material, a process which is probably RNA synthesis mediated by RNA polymerase. The macronuclei were thus highly purified, and retained a measure of biosynthetic activity.