Premium
Incorporation of Ribonucleoside Triphosphates into sRNA by Extracts of Tetrahymena pyriformis S *
Author(s) -
FITT P. S.
Publication year - 1966
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1966.tb01952.x
Subject(s) - tetrahymena pyriformis , ribonucleoside , biochemistry , transfer rna , tetrahymena , enzyme , rna , adenylate kinase , glycerol , biology , chemistry , gene
SYNOPSIS. Extracts of Tetrahymena pyriformis S in 50% glycerol contain an unstable enzyme capable of catalyzing the incorporation of ATP† or CTP into soluble RNA. Prior treatment of the sRNA with snake venom diesterase greatly enhances its ability to prime CTP incorporation. The properties of the enzyme suggest that it is an RNA‐adenylate (cytidylate) pyrophosphorylase.