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First Defined Media for Leptomonas spp. from Insects *
Author(s) -
GUTTMAN HELENE N.
Publication year - 1966
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1966.tb01926.x
Subject(s) - biology , thiolactone , methionine , botany , glycine , biochemistry , amino acid , stereochemistry , chemistry
SYNOPSIS. Defined media for Leptomonas collosoma, L. mirabilis , and L. sp. from Dysdercus are described. In addition to factors required by Crithidia spp., including a source of Crithidia factor, all 3 organisms require glycine. Leptomonas collosoma and L. sp. from Dysdercus require choline. All trypanosomatids thus far studied require calcium pantothenate but L. collosoma has an especially large requirement. Leptomonas mirabilis but not L . sp. from Dysdercus can synthesize methionine from homocysteine thiolactone during growth. Homocysteine thiolactone is toxic to L . sp. from Dysdercus . Methionine plus vitamin B 12 partly annul homocysteine thiolactone toxicity but only if glycine is also present.