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Survival of Tetrahymena pyriformis and Paramecium aurelia Following Freezing *
Author(s) -
WANG GUANGTSAN,
MARQUARDT WILLIAM C.
Publication year - 1966
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1966.tb01881.x
Subject(s) - paramecium aurelia , tetrahymena pyriformis , biology , population , cryopreservation , paramecium , dimethyl sulfoxide , tetrahymena , food science , zoology , chemistry , biochemistry , embryo , fishery , demography , organic chemistry , sociology
SYNOPSIS.Tetrahymena pyriformis strains E, A‐136 31C and IMT II survived freezing in 10% dimethylsulfoxide when the temperature was lowered to freezing at 4.5 C/min. Survival was then obtained for at least 128 days by lowering the temperature rapidly to 95°C. Of the 3 strains, T. pyriformis IMT II was most resistant to the effects of freezing. Its volume averaged about half that of either of the other strains and may have contributed to the differences in survival. In addition to differences among strains, a medium relatively low in the concentration of nutrients, a culture nearing peak population, and a rate of cooling of 4.5 C/min, all gave best survival. Paramecium aurelia regained motility after being frozen in 6 to 7.5% dimethylsulfoxide for as long as 7 days at either –27 or –196 C, but cultures were obtained only after storage for 20 min at –27 C. A concentration of 6 to 7.5% dimethyl‐sulfoxide, cooling at 4.5 C/min, and culture media containing Aerobacter aerogenes or composed of a commercially available composition were all required for survival of P. aurelia.