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Prolonged Culture of the Voracious Flagellate Peranema in Antioxidant‐Containing Media *
Author(s) -
ALLEN JAMES R.,
LEE JOHN J.,
HUTNER S. H.,
STORM JACK
Publication year - 1966
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1966.tb01877.x
Subject(s) - butylated hydroxytoluene , lecithin , ergosterol , chemistry , linoleic acid , food science , agar , antioxidant , sterol , cholesterol , organic chemistry , chromatography , fatty acid , biochemistry , biology , bacteria , genetics
SYNOPSIS.Peranema trichophorum remained vigorous at least a year in milk‐lecithin media and 3 months in a nearly defined autoclavable biphasic agar medium fortified with the fat‐soluble antioxidant butylated hydroxytoluene. The only undefined substance in the present “defined” medium is crude soybean lecithin; 0.001% lecithin suffices in the presence of a mixture of long‐chain saturated and unsaturated fatty acids. Linoleic acid may be indispensable. Histidine is an absolute requirement as well as a favored substrate. Cholesterol, not ergosterol, satisfied the sterol requirement. Voracity is retained in these media as shown by engorgement on plastic latex particles.