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Effect of pH of the Medium on the Availability of Chelated Iron for Chlamydomonas mundana
Author(s) -
MACIASR F. M.
Publication year - 1965
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1965.tb03248.x
Subject(s) - chelation , chlamydomonas , chemistry , metal , phosphate , inorganic chemistry , nuclear chemistry , biochemistry , organic chemistry , mutant , gene
SYNOPSIS. Nutritional and cultural factors influenced growth of Chlamydomonas mundana at different H‐ion concentrations. Growth at acid pH (5.5–6.0) primarily depended on the acetate and chelated iron concentration and on the method of preparation of the medium. The organism had increased Na, Ca, and Mn requirements at an acid pH. Sensitivity to increased levels of phosphate was greater at low than at high pH. The requirement for chelated as compared with non‐chelated Fe was qualitatively easier to demonstrate at low pH. The procedure followed in preparing the growth media was more important with higher levels of chelator and Fe and with media of low pH. All 6 synthetic chelators studied were more effective, as measured by growth, if combined with iron before addition to the media. At high pH (7.0–7.5), the organism was much more adept at utilizing over‐chelated Fe. This latter finding, and the greater sensitivity at low pH to trace‐metal imbalance in ill‐prepared media, indicate that the organism absorbs Fe much more efficiently at neutral or slightly alkaline pH.