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Glucose‐6‐phosphate Dehydrogenase and Reduced Glutathione in Malaria‐Infected Erythrocytes ( Plasmodium lophurae and P. berghei *
Author(s) -
SHERMAN IRWIN W.
Publication year - 1965
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1965.tb03231.x
Subject(s) - plasmodium berghei , parasitemia , glutathione , biology , enzyme , glucose 6 phosphate dehydrogenase , malaria , microbiology and biotechnology , dehydrogenase , virology , biochemistry , plasmodium falciparum , immunology
SYNOPSIS. Glucose‐6‐phosphate dehydrogenase activity and the level of reduced glutathione were assayed in host‐parasite interactions in which the malaria parasites preferentially invade: 1. mature erythrocytes ( Plasmodium lophurae in the duck) and 2. reticulocytes ( P. berghei in the mouse). Assay for glucose‐6‐phosphate dehydrogenase was by the dye decolorization test of Motulsky and Campbell. In P. berghei infected mouse erythrocytes with parasitemias ranging from 28–78% there was no deficiency in enzyme except at the 78% level. Similarly, P. lophurae ‐infected duck erythrocytes with parasitemias of 29–114% showed no enzyme deficiency except above the 80% level. No linear relationship existed between parasitemia and enzyme level. The reduced glutathione stability test of Beutler in duck erythrocytes infected with P. lophurae in the range 27–67% showed no glutathione instability.