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Biochemical Studies on Irradiated Protozoa. II. The Deamination of Deoxycytidine and Deoxycytidylic Acid by Tetrahymena pyriformis W *
Author(s) -
WINICUR SANDRA,
ROTH JAY S.
Publication year - 1965
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1965.tb01832.x
Subject(s) - tetrahymena pyriformis , deamination , deoxycytidine , enzyme , tetrahymena , biochemistry , chemistry , phosphate , cytidine deaminase , biology , chemotherapy , gemcitabine , genetics
SYNOPSIS. The high speed supernatant fraction obtained from cell‐free preparations of Tetrahymena pyriformis W contains an enzyme that deaminates deoxycytidine (dC) readily, but there is no evidence for a specific dCMP deaminase. The supernatant fraction also contains an active dCMP phosphatase which is inhibited by high phosphate concentration. dC deaminase in declining order deaminates BrdC, IdC, dC, MedC and dCMP. The enzyme does not appear to have a cofactor and is unaffected by Mg† and EDTA. It exhibits a broad pH optimum. Irradiation with 570,000 r of γ‐rays did not markedly affect the activity of dC, dG or dA deamination by Tetrahymena high speed supernatant fraction.