Premium
Comparative Metabolism of C 14 ‐Glucose in Two Strains of Trypanosoma cruzi *
Author(s) -
MANCILLA R.,
NAQUIRA G.
Publication year - 1964
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1964.tb01790.x
Subject(s) - pentose phosphate pathway , trypanosoma cruzi , biochemistry , metabolism , strain (injury) , carbohydrate metabolism , chemistry , d glucose , pentose , carbohydrate , hexose , metabolic pathway , biology , glycolysis , enzyme , fermentation , parasite hosting , world wide web , computer science , anatomy
SYNOPSIS. The Tulahuen and Peruvian strains of Trypanosoma cruzi have been compared as to their ability to metabolize C 14 ‐glucose uniformly labeled, or labeled in carbons 1, 2, or 6. Most of the radioactivity from uniformly labeled glucose is incorporated into organic acids in both strains, but significant radioactivity is also recovered in CO 2 . The Tulahuen strain has a slightly higher rate of glucose consumption, and the rate of incorporation of uniformly labeled glucose‐C 14 into CO 2 is also higher. More radioactivity is incorporated into proteins from uniformly labeled glucose‐C 14 in the Tulahuen strain, but the radioactivity recovered in total organic acids is fairly similar in both cases. On the basis of the rates of C 14 O 2 production from C 14 ‐glucose specifically labeled in carbons 1, 2, or 6, as well as the rates of incorporation into glycerol from uniformly labeled C 14 ‐glucose and glucose‐6‐C 14 , it is considered that the reactions of the pentose phosphate pathway play a more important role in the Tulahuen strain. The pentose phosphate pathway accounts for 41.4% of the glucose catabolized by the Tulahuen strain compared to only 27.9% in the Peruvian strain. It is suggested that differences in the participation of alternative pathways of carbohydrate metabolism may have some relationship to pathogenicity.