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Studies of Sulfate Utilization by Algae. 3. Products Formed From Sulfate by Euglena *
Author(s) -
Goodman Nelson S.,
Schiff Jerome A.
Publication year - 1964
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1964.tb01732.x
Subject(s) - euglena , iodoacetamide , sulfate , cysteine , glutathione , chromatography , cystine , chemistry , biochemistry , euglena gracilis , acetamide , methionine , algae , amino acid , biology , organic chemistry , botany , chloroplast , gene , enzyme
SYNOPSIS. Sulfate‐S 35 uptake by intact Euglena cells, as well as incorporation of sulfate‐S 35 into the insoluble fractions of the cells, was partially inhibited by adding iodoacetamide simultaneously or ten minutes before radioactive sulfate. The identification of S‐cysteine acetamide and S‐glutathione acetamide showed the presence of glutathione and cysteine in the soluble pool of Euglena but methionine, homocysteine, and S‐adenosylmethionine were not found during these experiments. An unidentified radioactive peak was eluted by 2 n HCl during column chromatography of the soluble fraction of Euglena cells. The major compound in this peak was identified as L‐cystine on the basis of its infra‐red spectrum, paper and column chromatographic characteristics and optical rotation. The use of iodoacetamide permitted the independent demonstration of cysteine and cystine in the soluble pools by eliminating oxidation artifacts.