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Isolation and Characterization of Deoxyribonucleic Acid of Two Species of Trichomonas Donne *
Author(s) -
Mandel M.,
Honigberg B. M.
Publication year - 1964
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1964.tb01730.x
Subject(s) - nucleic acid , isolation (microbiology) , dna , thymine , biochemistry , centrifugation , chemistry , biology , microbiology and biotechnology
SYNOPSIS. A modification of the method of Marmur permits the ready isolation of native deoxyribonucleic acid (DNA) from some strains of Trichomonas vagtnalis and T. gallinae. Analysis of the purified DNA by thermal denaturation and density gradient centrifugation shows both to be quite rich in adenine plus thymine and to display considerable compositional heterogeneity. Analyses for dry weight, total nitrogen, and amounts of the nucleic acids are also given.