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The Biology of Trypanosoma ranarum (Lankester, 1871). III. Localization of Acid and Alkaline Phosphatases in Flagellates Grown at Different Temperatures *
Author(s) -
LEHMANN DONALD L.
Publication year - 1963
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1963.tb01695.x
Subject(s) - alkaline phosphatase , incubation , enzyme , acid phosphatase , biochemistry , cytoplasm , chemistry , phosphatase , sulfide , biology , organic chemistry
SYNOPSIS. Alkaline (pH 8.4) and acid (pH 5.4) phospha‐tases were demonstrated in the cytoplasm of culture forms of Trypanosoma ranarum by the naphthol‐AS methods. The enzymes were in both slender and pear‐shaped flagellates, and variaticn of incubation temperature (10°, 31° and room temperature) did not adversely influence the appearance of the chromogenic granules. Enzyme determinations by sulfide methods indicated that alkaline phosphatase was concentrated in the nuclear area. Acid phosphatase could not be found by this method.