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Development of EDTA Media for the Rapid Growth of Chlamydomonas mundana
Author(s) -
MACIASR F. M.,
EPPLEY R. W.
Publication year - 1963
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1963.tb01670.x
Subject(s) - chelation , manganese , trace metal , metal , titration , growth rate , chemistry , chlamydomonas , magnesium , recovery rate , inorganic chemistry , nuclear chemistry , chromatography , biochemistry , mathematics , geometry , organic chemistry , mutant , gene
SYNOPSIS. An empirical method is used to determine mixtures of the chelating agent, EDTA (ethylenediamine tetraacetate), and trace metals which allow maximum growth rates of Chlamydomonas mundana under optimum environmental conditions. In this method which resembles an immunological titration one trace metal (Ca, Fe, Mn or Zn), in increasing concentrations, is used to “saturate” a standard amount of EDTA. Other required trace metals are added in a range of minimum concentrations. Extent of growth with each mixture is evaluated after 24 hours of incubation. Mixtures allowing greatest growth are then used to evaluate growth rate. Media with manganese as the EDTA‐saturating trace metal allow most rapid growth rates equivalent to 11.0 doublings per day. Magnesium as the EDTA‐saturating metal does not allow growth, suggesting that Mg does not displace other required trace metals from EDTA‐complex. Since the mixtures allowing maximum growth rate are “underchelated” it appears that the free metals rather than the metal‐chelate complexes are utilized by the alga in rapid growth.