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Production of a Vitamin B 12 Compound by Tetrahymenids *
Author(s) -
ERWIN JOSEPH A.,
HOLZ GEORGE G.
Publication year - 1962
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1962.tb02608.x
Subject(s) - tetrahymena pyriformis , euglena gracilis , euglena , biology , escherichia coli , vitamin , food science , chemistry , biochemistry , tetrahymena , gene , chloroplast
SYNOPSIS. Production of a “vitamin B 22 ‐like compound” by Tetrahymena pyriformis mating type II, variety 1, and 3 other tetrahymenids was demonstrated. The compound produced by T. pyriformis was nutritionally active for Escherichia coli and Lactobacillus leichmannii and inert for Ochromonas malhamensis and Euglena gracilis , despite use of a variety of the standard methods for liberating bound B 12 . By dry‐weight it is present in 1 part in 30 million. Its chromatographic and electrophoretic properties are described.