Premium
A Cytochemical Investigation of the Nuclear Changes in the Cyst Cycle of the Ciliate Telotrochidium henneguyi. *
Author(s) -
DODD EVERETT E.
Publication year - 1962
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1962.tb02587.x
Subject(s) - macronucleus , ciliate , nucleolus , biology , micronucleus , paramecium , feulgen stain , microbiology and biotechnology , nucleus , biochemistry , micronucleus test , genetics , chemistry , dna , organic chemistry , toxicity
SYNOPSIS. In a cytochemical study of nuclear changes during encystment and excystment of Telotrochidium henneguyi , the micronucleus was not observed to undergo alterations to correspond with the cyst cycle. The macronucleus maintained its gross structural integrity but showed internal reorganization of its nucleic acids coinciding with encystment and excystment of the ciliate. Nucleoli forming in the macronucleus of early precyst trophozoites first appeared as Feulgen‐ or methyl greenpositive clumps. These increased in size, became homogeneous, accumulated nucleolar substances, and assumed a Feulgennegative or pyroninophilic character. As the nucleoli developed, the remaining macronuclear constituents imparted a variegated pattern to the macronucleus. After excystment the nucleoli disappeared and the variegated pattern in the macronucleus was lost. Nucleoli were lacking in the macronucleus of trophozoites taken from the logarithmic phase of the growth curve.