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Excystment Ability, Respiratory Metabolism, and Ribonucleic Acid Content in Two Types of Resting Cysts of Colpoda cucullus O. F. Miiller
Author(s) -
PIGON A.,
EDSTRÖM J. E.
Publication year - 1961
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1961.tb01212.x
Subject(s) - respiration , biology , metabolism , oxygen , respiratory system , methylene blue , rna , bacteria , nucleic acid , microbiology and biotechnology , biochemistry , zoology , chemistry , anatomy , paleontology , organic chemistry , photocatalysis , gene , catalysis
Colpoda cucullus forms two kinds of resting cysts: stable cysts which withstand freezing and need 24 hours for activation, and unstable cysts which do not survive freezing, but are activated within 3‐4 hours. ImM 2,4‐dinitrophenol has no effect on stable cysts, and increases the respiration rate of unstable cysts by 100%. 1 mM methylene blue increases oxygen consumption in both stable and unstable cysts, by 200% and 400% resp. The results suggest that the inhibition of metabolism is more complete in stable cysts. These differences are not correlated with nucleic acid level, as both kinds of cysts contain approximately equal amounts of RNA.