Premium
Accumulation of Co 58 ‐Vitamin B 12 by Euglena gracilis *
Author(s) -
SEKHARA VARMA T. N.,
ABRAHAM ANNIE,
HANSEN I. A.
Publication year - 1961
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1961.tb01206.x
Subject(s) - euglena gracilis , intracellular , euglena , differential centrifugation , centrifugation , membrane , biophysics , biochemistry , chemistry , biology , chloroplast , gene
Some conditions for optimum accumulation of vitamin B 12 by resting Euglena gracilis cells were studied. Accumulation involves intracellular binding, as confirmed by disruption of the cells and differential centrifugation. At least 70% of the intracellular B 12 was attached to sedimentable particles, apparently to the mitochondrial and cell wall‐membrane fractions. The latter contains a heat‐labile substance which rapidly binds B 12 .