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Oxidative Assimilation of Acetate by Euglena . Carbon Balance and Effects of Ethanol *
Author(s) -
DANFORTH WILLIAM F.
Publication year - 1961
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1961.tb01198.x
Subject(s) - euglena gracilis , chemistry , ethanol , assimilation (phonology) , euglena , oxidative decarboxylation , carbon fibers , oxidative phosphorylation , carbon 14 , citric acid cycle , biochemistry , decarboxylation , food science , organic chemistry , metabolism , botany , biology , catalysis , linguistics , philosophy , materials science , physics , chloroplast , quantum mechanics , composite number , composite material , gene
Oxidative assimilation of acetate by Euglena gracilis was studied using methyl‐ and carboxyl‐labeled radioactive acetate. Of the total carbon utilized, 40% was oxidized to CO 2 and 60% was assimilated; 20% of the CO 2 produced was derived from the methyl carbon, and 80% from the car‐boxyl carbon of acetate. Seventy % of the carbon assimilated was derived from the methyl carbon and 30% from the carboxyl. The results suggest that assimilation involves decarboxylation of Krebs‐cycle oxalacetate and assimilation of the resulting pyruvate. In the presence of ethanol a greater proportion of the acetate carbon is assimilated, though the total acetate consumption is almost unchanged.