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Electron Microscopy of Nuclear and Cytoplasmic Events during Division in Tetrahymena pyriformis Strains W and HAM 3 *
Author(s) -
ROTH L. E.,
MINICK O. T.
Publication year - 1961
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1961.tb01174.x
Subject(s) - macronucleus , tetrahymena pyriformis , tetrahymena , interphase , nucleolus , biology , cell division , micronucleus , mitosis , microbiology and biotechnology , prophase , cytoplasm , strain (injury) , nucleus , endoplasmic reticulum , electron microscope , micronucleus test , genetics , ciliate , cell , chemistry , anatomy , meiosis , physics , organic chemistry , toxicity , gene , optics
Amicronucleate (strain W) and micronucleate (strain HAM 3) Tetrahymena pyriformis have been studied by light and electron microscopy. Numerous interphase and individually‐sectioned organisms in division stages have been examined. In macronuclei of the amicronucleate organisms (strain W) the time of division is characterized by a rather marked chromatin condensation, by morphological changes in nucleoli, and by the presence of dense granules. In macro‐nuclei of the micronucleus‐possessing organisms (strain HAM 3) little alteration of the interphase morphology is observed at division. Mitochondrial changes and an increase in the amount and complexity of the endoplasmic reticulum are observed in division in the amicronucleate strain. These results show that macronuclear division is more complex than a simple “pinching‐in‐two” and that loss of the micronucleus may alter the mitotic events of the macronucleus.