Premium
Isolation of Deoxyribonucleic Acid from Chlamydomonas reinhardi
Author(s) -
SCHWINCK ILSE
Publication year - 1960
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1960.tb00744.x
Subject(s) - chlamydomonas , lysis , dna , chemistry , isolation (microbiology) , biochemistry , chromatography , microbiology and biotechnology , biology , mutant , gene
SYNOPSIS. The purification procedure for native deoxyribonucleic acid (DNA) from the green alga Chlamydomonas reinhardi is described, using a modified detergent lysis, salt extractions, and alcohol precipitations. A tracer experiment with the addition of a bacterial transforming principle from Hemophilus influenzae demonstrated a recovery of mere than 30% of the bacterial transforming principle in an extensively purified DNA preparation.