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Comparative Physiological Studies on Four Species of Hemoflagellates in Culture. I. Endogenous Respiration and Respiration in the Presence of Glucose *
Author(s) -
ZELEDONt RODRIGO
Publication year - 1960
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1960.tb00722.x
Subject(s) - respiration , biology , endogeny , botany , biochemistry
SYNOPSIS. Endogenous respiration and respiration in the presence of glucose of the culture forms of Endotrypanum schaudinni, Leishmania enriettü, Schizotrypanum vespertilionis , and S. cruzi , have been compared in terms of cell number and nitrogen content. Growth curves for the 4 species at 26° C. showed that E. schaudinni and L. enriettü grow faster than the Schizotrypanum in a reproducible modification of Senekjie's diphasic medium. Respiration was studied manometrically using organisms harvested in the exponential phase of growth. Res‐piratory patterns at 30° C. and pH 7.2 are presented for each species. Low endogenous rates are characteristic of E. schaudinni and L. enriettii ; they are relatively high in both Schizotrypanum spp. The respiration of E. schaudinni is stimulated from 5.3 to 36.1 times by glucose; that of L. enriettii from 5.2 to 9.7 times and that of the Schizotrypanum from 1.9 to 5.7 times. Carbohydrate is of great importance for the survival and motility of E. schaudinni and L. enriettii , but plays a relatively minor role in Schizotrypanum.