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Identification of Free and Bound Amino Acids in Three Strains of Tetrahymena pyriformis using Paper Chromatography *
Author(s) -
WELLS CAROLYN
Publication year - 1960
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1960.tb00700.x
Subject(s) - tetrahymena pyriformis , strain (injury) , amino acid , isoleucine , paper chromatography , leucine , tetrahymena , biology , biochemistry , clone (java method) , arginine , alanine , valine , genetics , chemistry , gene , anatomy
SYNOPSIS. The free and bound amino acids of 3 strains of Tetrahymena pyriformis (variety 6) have been identified by filter paper chromatography. Twenty bound amino acids were qualitatively and quantitatively alike in each strain. The same 17 free amino acids were identified in all 3 strains; however, quantitative variations among the cultures were noted. Strain 002. an F 1 progeny clone obtained from a cross between strains EU 6000 and EU 6001, exhibited a combination of parental characteristics. The F 1 organisms resembled one parental strain, EU 6000, in the ratio of free arginine to alanine, and the other parental strain, EU 6001, in the ratio of free isoleucine to leucine. The possible genetic significance of these results is noted, and the value of chromatographic analysis as an aid to genetic study is discussed.