Premium
Non‐axenic and Axenic Growth of Vorticella microstoma
Author(s) -
FINLEY HAROLD E.,
McLAUGHLIN DAVID,
HARRISON DORIS M.
Publication year - 1959
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1959.tb03952.x
Subject(s) - axenic , axenic culture , biochemistry , biology , yeast extract , food science , microbiology and biotechnology , chemistry , chemically defined medium , bacteria , in vitro , fermentation , genetics
SYNOPSIS.Vorticella microstoma was grown non‐axenically and axenically at pH 6.4. Vorticellas were maintained indefinitely on Bacillus cereus in a medium composed of Proteose‐Peptone, Cerophyl, and the filtrate from boiled wheat kernels. Prolific growth occurred in 2‐membered cultures. A medium containing hydrolyzed gelatin, aqueous liver extract, yeast nucleic acid hydrolysate, glucose, and penicillin is recommended for axenic growth. The potential value of vorticellids as research tools is discussed together with metabolic implications of supplementing sterile Proteose‐Peptone broth with natural substances in particle form. The ineffectiveness of adding tryptophan, thiamine, glycine, and chelating agents to the axenic medium was considered. Refinements of the axenic medium are on trial.