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The Relation of the Stage of the Population Growth Curve to the Survival of Tritrichomonas foetus upon Freezing in the Presence of Glycerol *
Author(s) -
LEVINE NORMAN D.,
McCAUL WILLIAM E.,
MIZELL MERLE
Publication year - 1959
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1959.tb03936.x
Subject(s) - population , glycerol , maltose , zoology , sugar , biology , sucrose , food science , medicine , biochemistry , environmental health
SYNOPSIS. When grown in CPLM medium or in a similar medium containing glucose instead of maltose as its sugar, Tritrichomonas foetus , Strain O, was much more sensitive to injury when frozen to ‐21d̀ C. in the presence. of 1 m glycerol during the initial and logarithmic phases of its population growth curve than at its peak and for some time thereafter. In 7 experiments in which the population peak occurred an average of 28.1 hours after inoculation, the average culture age at which the protozoa first survived freezing was 20.3 hours, at which time the population had reached 52.6% of its peak. The optimum culture age for survival after 1 day of freezing averaged 37.7 hours at which time the population averaged 75.6% of its peak. The optimum culture age for survival after 7 to 15 days of freezing averaged 32.3 hours, at which time the population averaged 82.7% of its peak. Better survival upon freezing was obtained in those experiments in which the population peak was reached relatively rapidly.