Premium
Gel Diffusion Studies on the Antigens of Isolated Cellular Components of Paramecium *
Author(s) -
PREER J. R.,
PREER L. B.
Publication year - 1959
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1959.tb03932.x
Subject(s) - paramecium , antigen , biology , mitochondrion , paramecium aurelia , microbiology and biotechnology , diffusion , chemistry , biophysics , immunology , physics , thermodynamics
SYNOPSIS. A method for the isolation of trichocysts and cilia from whole paramecia is given. Trichocysts, cilia, mitochondria, and other particulates have been identified in homogenates and their numbers in different centrifugal fractions determined. Four antigens extractable from one strain of Paramecium have been studied by gel‐diffusion techniques: Antigen 1, heat‐stable, derived from trichocysts; antigen 2, not associated with any cellular particulate in homogenates; antigen 3, obtained in large quantities from the mitochondria; antigen 4, the antigen correlated with serotype, is derived mainly from the cilia and probably from the body wall and having the properties of a protein.