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Assay of Pteridines with Crithidia fasciculata *
Author(s) -
NATHAN HELENE A.,
HUTNER S. H.,
LEVIN H. L.
Publication year - 1958
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1958.tb02540.x
Subject(s) - crithidia fasciculata , folinic acid , crithidia , biochemistry , folic acid , conjugated system , chemistry , hydrolysis , pteridine , biology , organic chemistry , enzyme , botany , medicine , protozoa , genetics , colorectal cancer , cancer , polymer
SYNOPSIS. Detailed directions are given for the preparation of a simplified assay medium and of natural materials for assay of pteridines with Crithidia fasciculata. Unconjugated pteridines in natural materials which also contain conjugated pteridines (folic and folinic acids) can be assayed by destroying the conjugated pteridines with acid hydrolysis, which does not appreciably alter Crithidia‐active unconjugated pteridines. The widespread occurrence of unconjugated pteridines and their significance in such processes as photoreception are discussed.