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Studies on Trichomonads. I. The Metabolism of Tritrichomonas foetus and Trichomonads from the Nasal Cavity and Gecum of Swine
Author(s) -
Doran David J.
Publication year - 1957
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1957.tb02506.x
Subject(s) - tritrichomonas foetus , biology , raffinose , microbiology and biotechnology , galactose , lactose , fetus , biochemistry , sucrose , pregnancy , genetics
SUMMARY. The metabolism of Tritrichomonas foetus (strain, BP‐1) and trichomonads from the nasal cavity and cecum of swine was studied manometrically under similar experimental conditions. At pH 6.4, quantitative and qualitative differences were observed. The cecal (probably T. suis ) and nasal trichomonad used glucose, galactose, fructose, mannose, lactose, sucrose, raffinose, and trehalose. T. foetus used all except lactose and raffinose. All three were inhibited by iodoacetate and arsenite. T. foetus and the nasal form were significantly inhibited by fluoride and 8‐hydroxyquinoline, whereas the cecal trichomonad was not. At varied pH, all failed to oxidize Krebs' cycle intermediates. The amounts of oxygen consumed by T. foetus and the nasal trichomonad in the presence of lactate and pyruvate were at levels similar to those with disaccharides; the cecal trichomonad was indifferent toward both substances. Anaerobically, lactate and pyruvate increased the evolution of gas by all three trichomonads. Aerobic acid formation was demonstrated for all three forms. Anaerobically, metabolic CO 2 and gas(es) that were not absorbed by KOH were evolved by all three. Pure oxygen was inhibitory to glucose utilization and stimulatory to the endogenous respiration of all trichomonads; the nasal form was affected the least. The writer believes that the cecal trichomonad is different from T. foetus and the nasal trichomonad of swine. The relationship between the nasal trichomonad and T. foetus remains in doubt.