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Replacement of Serum for In Vitro Cultivation of Tritrichomonas foetus *
Author(s) -
Sanders Marvin
Publication year - 1957
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1957.tb02495.x
Subject(s) - tritrichomonas foetus , cholesterol , choline , in vitro , fetal bovine serum , fetus , microbiology and biotechnology , chemistry , potassium , biology , biochemistry , pregnancy , genetics , organic chemistry
SUMMARY. The combination Tween 80 and cholesterol replaced blood serum as a requirement for the cultivation of Tritrichomonas foetus. Choline + potassium glycerophosphate enhanced growth in the presence of Tween 80 + cholesterol. A method for ensuring adequate cholesterol suspension‐an essential factor for consistent growth‐is described.