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The Induction of Haploidy in Tetrahymena pyriformis Following X‐irradiation. 1
Author(s) -
ELLIOTT A. M.,
CLARK G. M.
Publication year - 1956
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1956.tb02453.x
Subject(s) - ploidy , biology , mating type , tetrahymena pyriformis , mating , tetrahymena , clone (java method) , genetics , nucleus , macronucleus , botany , microbiology and biotechnology , gene
SUMMARY. Vegetative clones of Tetrahymena pyriformis , variety 2, mating type II, were the recipients of various dosages of X‐rays from 10,000 to 400,000r. One clone from the 400,000 level (UM7X), when mated with non‐irradiated cells of various mating types in variety 2, failed to produce a migratory nucleus. A one‐way transfer from the normal to irradiated mate occurred, resulting in the production of haploid exconjugants. When haploids were crossed with diploids the former failed to produce a migratory nucleus. A one‐way nuclear transfer from the diploid to the haploid occurred resulting again in the production of haploid exconjugants. When two haploids were crossed a clone resulted which showed 80 to 90 percent amicronucleate forms. The mating type of the derived haploid is always that of the diploid parent. All derived haploids manifest physiological and morphological characteristics similar to those of the diploid except that the haploids possess a smaller micronucleus.