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A Simple Method for Paraffin and Plastic Embedding of Protozoa
Author(s) -
RUDZINSKA MARIA A.
Publication year - 1955
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1955.tb02421.x
Subject(s) - centrifugation , centrifuge , protozoa , tube (container) , chromatography , fixation (population genetics) , materials science , pellet , biology , chemistry , composite material , microbiology and biotechnology , biochemistry , physics , gene , nuclear physics
SUMMARY. To avoid multiple centrifugation and considerable losses of material in preparing protozoa for paraffin and plastic embedding a simple method has been worked out, requiring only two centrifugations, one before and one after fixation. During the second centrifugation, which is done at 700 g for 10 minutes, the organisms form a cohesive pellet which may be removed from the centrifuge tube by means of a fine spatula and handled as a piece of tissue through the whole process of dehydration and embedding.