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Homalozoon vermiculare (Stokes): II. Parapharyngeal Granules and Trichites *
Author(s) -
WEINREB STANLEY
Publication year - 1955
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1955.tb02401.x
Subject(s) - stain , chemistry , acetic acid , anatomy , nile red , staining , biophysics , biology , pathology , biochemistry , medicine , fluorescence , optics , physics
SUMMARY. The parapharyngeal granular masses of Homalozoon vermiculare (Stokes) are stainable in vivo with Janus green B. The reaction is the same in the case of similar granules which are scattered throughout the trunk region. It is, therefore, assumed that these granules are mitochondrial in nature. These same granules failed to stain with osmic acid or acetic‐carbol‐Sudan III although they took an intense peripheral stain when treated in vivo with very dilute Nile blue sulfate. This indicates that they probably have a peripheral layer of phospholipids which would be expected if they are mitochondrial in nature. The trichites, although not stained by lipid techniques, are stained by mercuric bromphenol blue which confirms their proteinaceous nature. They are swelled by Carnoy's acetic‐alcohol causing them to become less visible in situ . Trichites are found surrounding the cytostome and free‐floating throughout the endoplasm but have not been observed subpellicularly or within the ectoplasm. There are indications that they originate within the endoplasm and do not have a homogeneous structure.